A tryparedoxin-coupled biosensor reveals a mitochondrial trypanothione metabolism in trypanosomes
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چکیده
منابع مشابه
Mitochondrial Redox Metabolism in Trypanosomatids Is Independent of Tryparedoxin Activity
Tryparedoxins (TXNs) are oxidoreductases unique to trypanosomatids (including Leishmania and Trypanosoma parasites) that transfer reducing equivalents from trypanothione, the major thiol in these organisms, to sulfur-dependent peroxidases and other dithiol proteins. The existence of a TXN within the mitochondrion of trypanosomatids, capable of driving crucial redox pathways, is considered a req...
متن کاملRoles of trypanothione S-transferase and tryparedoxin peroxidase in resistance to antimonials.
The clinical value of antimonial drugs, the mainstay therapy for leishmaniasis, is now threatened by the emergence of acquired drug resistance, and a comprehensive understanding of the underlying mechanisms is required. Using the model organism Leishmania tarentolae, we have examined the role of trypanothione S-transferase (TST) in trivalent antimony [Sb(III)] resistance. TST has S-transferase ...
متن کاملThe parasite-specific trypanothione metabolism
Trypanosomes and leishmania are the causative agents of African sleeping sickness (Trypanosoma brucei), South American Chagas’ disease (T. cruzi) and other tropical diseases. All these parasitic protozoa have a unique thiol metabolism. Instead of the ubiquitous glutathione, the main non-protein thiol is trypanothione [bis(glutathionyl)spermidine] which is kept reduced by the flavoenzyme trypano...
متن کاملDepletion of the thioredoxin homologue tryparedoxin impairs antioxidative defence in African trypanosomes.
In trypanosomes, the thioredoxin-type protein TXN (tryparedoxin) is a multi-purpose oxidoreductase that is involved in the detoxification of hydroperoxides, the synthesis of DNA precursors and the replication of the kinetoplastid DNA. African trypanosomes possess two isoforms that are localized in the cytosol and in the mitochondrion of the parasites respectively. Here we report on the biologic...
متن کاملIron Metabolism in Pathogenic Trypanosomes
Trypanosomatids comprise a large group of flagellated unicellular protists with free-living and parasitic lifestyles. Several members of this family are widely known for being vertebrate pathogens of biomedical and veterinary importance. They belong to the order Kinetoplastida, which together with the groups Diplonemida (deep-see organisms) and Euglenida (photosynthetizing organisms) form the p...
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ژورنال
عنوان ژورنال: eLife
سال: 2020
ISSN: 2050-084X
DOI: 10.7554/elife.53227